Poster Session 8: Trauma and reconstruction| Volume 8, ISSUE 8, P646, September 2009

S117 In-vivo haemostatic effect of Ankaferd Bloodstopper in rat major renal trauma model: controlled trial of novel haemostatic agent

      Introduction and Objectives

      Major renal trauma is one of the most mortal condition which could cause death. Bleeding is the main reason of mortality. We used Ankaferd Bloodstopper (ABS) which is a medicinal product has been approved in the control of hemorrhage externally and dental surgery bleedings in Turkey to control the bleeding in renal trauma model and evaluated the efficacy of ABS.

      Material and Methods

      Twelve Wistar rats were divided into two groups. Group I (GI), control, Group II (GII), study group. Under general anesthesia, following the exposure of right kidney, 1 cm. deep incision was performed at the lower pole of kidney and 1cm2 tissue was resected. ABS solution was applied to resected area and compressed at least two minutes. Afterthat, bleeding control was evaluated. Time of bleeding control, number of ABS gout, live condition of rats following surgery were evaluated, at first month, sacrification was performed, macroscopic and microscopic features were determined.


      Mean time of bleeding control was 3.2±0.8 (2−4) min in GII, no difference with GI (p < 0.05). In each kidney, active haemostasis was provided with observing the aggregation unit of ABS onto the renal resected surface. In GII, active hemostasis was provided. Mean number of ABS gout was 6.0±1.1 (5−8). Glomerular necrosis was detected with higher rate in GI compared with GII. Erythrocyte aggregation was confirmed in GII. Calcification was formed significantly in GI compared GII (p < 0.05). ABS kidneys were all in a good shape especially nearly to resected area, however, gelatinous, redness and wealthy tissue were observed in a macroviews at transected kidney. There were no hematomas, urinomas and urine leakage. In microscopic evaluation (H.E.), giant cell reaction, acute inflammation, fibrosis, adhesion, tiroidization, fibroblast activation, calcification, fibrosis, glomerular necrosis, adhesion to adjacent organ were not detected while erythrocyte aggregation, cydherophage and microvascular proliferation were shown in each kidney.


      ABS is an effective agent to stop active major bleeding in renal trauma model. The effect of ABS to renal histopathology shows positive clues without inflammation, fibrosis and tissue damage, and erythrocyte aggregation, bleeding control mechanism of ABS, are also shown in kidney histopathologically.