Introduction and Objectives
To evaluate the preventive effects of Coenzyme Q10 (CoQ10) in ischemia/reperfusion (I/R) injury due to testicular torsion/detorsion (T/D).
Material and Methods
Adult male Wistar rats were divided into 3 groups of 7 each. One group underwent 1 h of testicular torsion and 4 h of detorsion; one received pretreatment with CoQ10 before detorsion; and one group underwent sham operation. At the end of the experiments, bilateral orchiectomies were performed. Lipid peroxidation products, inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), and apoptosis protease-activating factor 1 (APAF-1) were assessed in the testis.
Results
Testicular T/D caused a significant increase in lipid peroxidation products, iNOS, eNOS and APAF-1 expressions in ipsilateral testes (p < 0.001), but not in the contralateral testes. The animals treated with CoQ10 had a significant decrease in these parameters compared with T/D group (p < 0.01).
Conclusions
These data emphasize that CoQ10 administration before the reperfusion period of testicular torsion provides a significant decrease in testicular lipid peroxidation products, expressions of iNOS and eNOS and germ cell-specific apoptosis.
Article info
Identification
Copyright
© 2009 European Association of Urology. Published by Elsevier Inc. All rights reserved.
