Poster Session 6: External genital| Volume 8, ISSUE 8, P596, September 2009

N79 Coenzyme Q10 treatment reduces lipid peroxidation, inducible and endothelial nitric oxide synthases and germ cell-specific apoptosis in testicular ischemia/reperfusion injury

      Introduction and Objectives

      To evaluate the preventive effects of Coenzyme Q10 (CoQ10) in ischemia/reperfusion (I/R) injury due to testicular torsion/detorsion (T/D).

      Material and Methods

      Adult male Wistar rats were divided into 3 groups of 7 each. One group underwent 1 h of testicular torsion and 4 h of detorsion; one received pretreatment with CoQ10 before detorsion; and one group underwent sham operation. At the end of the experiments, bilateral orchiectomies were performed. Lipid peroxidation products, inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), and apoptosis protease-activating factor 1 (APAF-1) were assessed in the testis.


      Testicular T/D caused a significant increase in lipid peroxidation products, iNOS, eNOS and APAF-1 expressions in ipsilateral testes (p < 0.001), but not in the contralateral testes. The animals treated with CoQ10 had a significant decrease in these parameters compared with T/D group (p < 0.01).


      These data emphasize that CoQ10 administration before the reperfusion period of testicular torsion provides a significant decrease in testicular lipid peroxidation products, expressions of iNOS and eNOS and germ cell-specific apoptosis.